Estimation of transmittance spectra from multiband micrographs of fungi and its application to segmentation of conidia and hyphae

Masaru Takeya, Norimichi Tsumura, Hideaki Haneishi, and Yoichi Miyake

Department of Information and Image Sciences, Chiba University 1-33, Yayoi-cho, Inage-ku, Chiba 263-8522, Japan

APPLIED OPTICS Vol. 38, No. 16 (June 1999)3644-3650

Abstract
Transmittance spectra of fungi were estimated from seven-band optical micrographs. An optical microscope adjusted with a single-chip CCD camera with seven band filters was used for image acquisition. The Wiener method was applied to estimate the transmittance spectra of five species that belong to one genus of fungi. The Wiener estimation operator was calculated from transmittance spectra of 16 color transparencies and the corresponding camera responses. The estimated transmittance spectra were used for segmentation of conidia and hyphae in fungal optical micrographs; then the competitive learning in an artificial neural network was applied to the segmentation.

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