Masaru Takeya, Takehiro Matsuda
, Masao Iwamoto,
Norimichi Tsumura, Toshiya Nakaguchi, and Yoichi MiyakeP
Division of Genome and Biodiversity ResearchT,
National Institute of Agrobiological Sciences,
2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
Graduate School of Science and Technology, Chiba University,
1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan
Division of Plant SciencesT, National Institute of Agrobiological Sciences,
2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
OPTICAL REVIEW Vol.14, No.2, pp.97-104(2007).
Abstract
We propose a technique for estimating gene expression values for duplicated
data on cDNA microarrays. In the scatter plots, the distribution is constructed
from a mixture of normal two-dimensional distributions, which represent
fluctuations in gene expression values due to noise. An EM algorithm is
used for estimating the modeling parameters. The probability that duplicated
data is shifted by noise is calculated using Bayesian estimation. Six data
sets of rice cDNA microarray assays were used to test the proposed technique.
Genes in the data sets were subjected to clustering based on probability
of true value. Clustering successfully identified candidate genes regulated
by circadian rhythms in rice.
Full paper ( to appear )
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